Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 10;13:5986. doi: 10.1038/s41467-022-33483-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Cryo-electron tomogram of an MRT68921-treated, PV-infected cell containing cytoplasmic virion arrays. b Segmentation of the tomogram in (a). Virions are represented by their subtomogram averages. Colors are as in Fig. 1b. c Percentage of cells containing virus arrays in DMSO and MRT68921-treated cells as measured from freeze-substituted sections. d–g Several examples of virion recruitment to ALMs in tomograms of MRT68921-treated, PV-infected cells. h Time course of PV release from MRT68921 and DMSO-treated cells. Error bars represent the means of biological triplicates ± SEM. i Western blot analysis of extracellular vesicles (EVs) harvested at 8 h p.i. from infected cells treated or not with MRT68921. j Slice through a cryo-electron tomogram recorded at the plasma membrane (PM) of a non-FIB-milled MRT68921-treated, PV-infected cell. k–m Magnified views of PV-containing released vesicles indicated in (j). n 3D segmentation of the tomogram in (j). PM (yellow), RNA-loaded virions (red), extracellular vesicles (purple), luminal densities (orange). Scale bars: 100 nm.