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. 2022 Oct 10;11(1):61. doi: 10.1038/s41389-022-00436-0

Fig. 3. Differential TF-SE interactions correspond with glycolysis reprogramming.

Fig. 3

A Schematic of the approach used to identify TFs with potential molecular function. B The LASSO coefficient profiles for the prediction of SHCs. C The motifs in the promoters of SE-regulated genes. D, E Kaplan–Meier survival curves for predicted clusters (according to the 3-TF index model) displaying overall survival in TCGA cohort and GSE37745. F Correlation plots showing the correlation between glycolysis score and 3-TF index value in 78 LUAD cell lines from the CCLE database. G, H There were tendencies of high glucose uptake and lactate excretion in cell lines with high 3-TF index than in cell lines with low 3-TF index. I, J Effect of 2-DG on the confluence of LUAD cell lines with high 3-TF index and low 3-TF index. Extracellular flux assays using Seahorse (K, L), EdU assay (M) and clone formation assay (N) showing that cell lines with high 3-TF index (DV90, H1975) have higher 2-DG sensitivity than cell lines with low 3-TF index (A549, A427). Asterisks indicate statistical significance; *P < 0.0.5; **P < 0.01; ***P < 0.001.