Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 10;13:5859. doi: 10.1038/s41467-022-33605-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 8 — a Effect of 1 week LIM on the expression of 43 collagen genes in C57BL6J mouse sclerae (NL: n = 8, −30D: n = 7). No Lens group was assigned a value of 1.0. *p < 0.05 compared to each No Lens group; Student’s two-tailed t-test (Exact p values are as follows. 4a2: p = 0.023, 4a3: p = 0.001, 6a1: p = 0.008, 6a2: p = 0.027, 6a5: p = 0.024, 7a1: p = 0.009, 8a1: p = 0.041, 8a2: p = 0.024, 11a2: p = 0.022, 12a1: p = 0.048, 15a1: p = 0.036, 18a1: p = 0.038). b Effect of 3 weeks LIM on the expression of 43 collagen genes in C57BL6J mouse sclerae (NL: n = 8, −30D: n = 7). No Lens group was assigned a value of 1.0. *p < 0.05 compared to each No Lens group; Student’s two-tailed t-test (Exact p values are as follows. 1a1: p = 0.012, 4a3: p = 0.009, 24a1: p = 0.017). c 4-PBA instillation cancels LIM-induced decrease in COL1A1 protein expression in sclerae (n = 4, each sample pooled 3 sclerae). NL: No Lens control, −30D: minus 30 D lens-wearing eye. d The effect of LIM and 4-PBA instillation on Col1a1 mRNA expression in sclerae (n = 6, respectively). The p values were determined by Generalized Estimating Equations. e 4-PBA instillation cancels LIM-induced increase in Col4a3, Col8a2, Col11a2, and Col15a1 mRNA expression evaluated by qPCR in sclerae (n = 6 per group); The p values were determined by two-tailed Generalized Estimating Equations. f Transmission electron microscopy images of sclerae collagen fiber in No lens control (NL) and −30 D lens worn (−30D) sclera of PBS or 4-PBA instilled C57BL6J mouse. Representative images of three biologically independent samples; scale bar, 200 nm. g 100% stacked bar charts of scleral collagen fiber area (n = 3 biologically independent samples). Five images from one sclera. Fiber area was measured using ImageJ software. Source data are provided as a Source Data file.