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(A) A diagram of a hybrid differentiation paradigm employing the same regionalizing steps as directed differentiation while inducing specific transcription factors (TFs), miRNA, or shRNA to quickly induce or refine cell type during neural specification. (B) An example of a potential way to generate V2a neurons using the hybrid approach in 2A. TFs, transcription factors; miRNA, micro ribonucleic acid; shRNA, short hairpin ribonucleic acid; (Created with BioRender.com).
