TABLE 1.

Comparison of the averaged values of DNA purity and concentration yielded by different DNA extraction methods and estimated execution time for each method

Method		Onychomycosis models	DNA purity (Mean ± SE)	DNA concentration (Mean ± SE) μg/ml	Time estimation
Extraction	Purification
Method 1
Steel bullet + lysis buffer	Phenol chloroform	Model 1	1.91 ± 0.10	244 ± 31.27	≃80 min
		Model 2	1.84 ± 0.52	490 ± 54.82
Method 2
Freezing + steel bullet+ lysis buffer	Phenol chloroform	Model 1	1.74 ± 0.01	366 ± 49.69	≃140 min
		Model 2	1.93 ± 0.04	884 ± 83.56
Method 3
Steel bullet	Commercial kit	Model 1	1.77 ± 0.09	169.2 ± 27.94	≃65 min
		Model 2	1.3 ± 0.18	440 ± 50.82
Control method
Glass beads + lysis buffer	Phenol chloroform	Model 1	1.86 ± 0.12	117 ± 32.48	≃80 min
		Model 2	1.83 ± 0.05	262 ± 39.75