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. 2022 Sep 27;39(10):msac195. doi: 10.1093/molbev/msac195

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© The Author(s) 2022. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — Isolation of S. latifolia GSF gene and its homology to A. thaliana CLV3 gene. (A) Photographs of flowers of the male, female, and hermaphroditic mutant S. latifolia plants. Bar 1 cm. (B) Scheme of the GSFY gene isolation. (C) Genomic PCR analysis of the GSF candidate in male, female, and 11 hermaphroditic mutants (EGP4, EGP5, EGP6, EGP8, EGP9, EGP10, EGP11, EGP12, EGP13, EGP14, and EGP15). All mutants showed no amplification by the candidate specific primers. (D) Deduced amino acid sequence homology between Y-linked GSF (GSFY), X-linked GSF (GSFX), and A. thaliana CLV3 genes. The conserved CLE domain is underlined. (E) Homology of deduced CLE domain sequences between GSFY, GSFX, CLV3, and its null allele (clv3-1 or clv3-5); CLV3m.