. 2001 Dec;183(23):6727–6732. doi: 10.1128/JB.183.23.6727-6732.2001

TABLE 1.

Plasmids used in this work

Plasmid	Relevant properties^a	Source or reference
E. coli plasmids
pBluescript KS(+)	Multiple cloning site; Ap^r	Stratagene
pGEM-T-Easy	Used for cloning of PCR products and for the construction of a tatC deletion mutant; Ap^r	Promega
pBSKAN	pBluescript KS(+) derivative with an inserted neo gene; Kan^r Ap^r	This work
pMW18	pBluescript KS(+) derivative containing pre-TorA-23K; Ap^r	7
pBS-CBSS	pBluescript KS(+) derivative containing the S. venezuelae vsi promoter	23
Streptomyces plasmids
pIJ486	Multiple cloning site; Tsr^r	42
pVsiTor23K	pIJ486 derivative; contains pre-TorA-23K after the S. venezuelae vsi promoter; Tsr^r	This work
pIJ702	contains the S. antibioticus melC operon; Tsr^r	18
E. coli/Streptomyces shuttle vectors
pFD666	Kan^r	13
pGM160	temperature sensitive replicon; Ap^r Tsr^r	28
pGMΔtatC	pGM160 derivative used to knock out S. lividans tatC; Kan^r Tsr^r	This work
Integrative vectors containing φC31 att-locus
pSET152	Integrative plasmid; Apr^r	4
pSETtatC	pSET152 derivative containing S. lividans tatC	This work

Ap^r, ampicillin resistance; Apr^r, apramycin resistance; Kan^r, kanamycin resistance; Tsr^r, thiostrepton resistance.