Skip to main content
. 2022 Oct 10;12:439. doi: 10.1038/s41398-022-02208-1

Fig. 2. Age-linked reductions in LXA4 result in cognitive impairment.

Fig. 2

a, b Levels of lipoxin A4 (LXA4) in plasma (a) and brain (b) of young (3 month old) or aged (12 month old) Swiss mice. (Plasma: N = 12 for 3 mo, N = 15 for 12 mo; Brain: N = 7 for 3 mo, N = 15 for 12 mo mice). c Step-down latency of young or aged mice in the inhibitory avoidance task to assess short-term memory (STM: 1 h after training; N = 20 for 3 mo; N = 15 for 12 mo mice). d Number of trials required for each mouse to reach the criteria during the training session in the inhibitory avoidance fear task (N = 23 for WT; N = 13 for 5-LOX−/−). e, f Step-down latency of 5-LOX−/− or WT mice (3 month old) in the inhibitory avoidance task to assess short-term (e; STM: 1 h after training; N = 7 WT and N = 15 5-LOX−/− mice) or long-term memory (f; LTM: 24 h after training; N = 23 for WT; N = 13 for 5-LOX−/−). For a, b, and d, two-tailed unpaired Student’s t-test. For c, e, and f, two-tailed unpaired Mann–Whitney (*p < 0.05; ****p < 0.0001). Graphs show means ± standard error of the mean (SEM). Each dot represents an individual. g Fear memory extinction assessed by step-down latency of 5-LOX−/− or WT after repeated test sessions 12–15 days after original conditioning session (N = 10 WT and N = 10 5-LOX−/− mice). Repeated measures one-way ANOVA with Šidák post hoc test (*p < 0.05). Graphs show means ± SEM.