Fig. 3. Single channel study and molecular dynamic simulation of nanopores.

a Single channel current traces of Ch-C1 recorded in symmetric (cis chamber = trans chamber = 1 M KCl) baths, the red lines refer to the mean current values for plotting current-voltage (I-V) curve for obtaining the ion conductance (γ) and pore size (1.72 nm) for Ch-C1. b I-V curve and estimated pore size (0.94 nm) for Ch-C4. Data are represented as mean current values ± half width at half maximum. n  =  3 independent experiments. c Molecular dynamics-simulated pore-forming ensembles of different sizes made up of channel (Ch-C1 or Ch-C4 in yellow) and Ch (in blue) molecules in 1:1 molar ratio. d An alternative self-assembling possibility involving Ch-C1 and Ch in 1:2 molar ratio to generate a pore of 1.40 nm.