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. 2022 Oct 10;13:5973. doi: 10.1038/s41467-022-33671-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a qRT-PCR analysis of Ifnb, Il6 and Ip10 mRNA in Arih1^fl/fl and Lyz2-Cre;Arih1^fl/fl BMDCs infected with HSV-1 for 0–8 h or transfected with ISD45, HSV60 or DNA90 (1 μg) for 8 h. b qRT-PCR analysis of Ifnb, Il6 and Ip10 mRNA in Arih1^fl/fl and Lyz2-Cre;Arih1^fl/fl BMDCs infected with SeV for 0–8 h or transfected with poly(I:C) (1 μg) or cGAMP (1 μg) for 0–4 h. c ELISA analysis of IFN-β and IL-6 in the supernatants of Arih1^fl/fl and Lyz2-Cre;Arih1^fl/fl BMDCs infected with HSV-1 or SeV for 12 h, or transfected with cGAMP (1 μg) for 12 h. d Immunoblot analysis of total and phosphorylated (p-) IRF3, TBK1 and p65, and ARIH1 and GAPDH in Arih1^fl/fl and Lyz2-Cre;Arih1^fl/fl BMDCs infected with HSV-1 (left) or SeV (right) for 0–8 h. e Flow cytometric analysis (upper flow charts) and fluorescent microscopy imaging (lower images) of the replication of H129-G4 (MOI = 0.5) in Arih1^fl/fl and Lyz2-Cre;Arih1^fl/fl BMDCs that were left uninfected or infected with H129-G4 for 1 h followed by twice PBS wash and culture in full medium for 24 h. Numbers adjacent to the outlined areas indicate percentages of GFP⁺ BMDCs. Scale bars represent 200 μm. f qRT-PCR analysis of HSV-1 UL30 mRNA in Arih1^fl/fl and Lyz2-Cre;Arih1^fl/fl BMDCs (upper graph) and plaque assays analyzing HSV-1 titers in the supernatants of Arih1^fl/fl and Lyz2-Cre;Arih1^fl/fl BMDCs (lower graph) infected with HSV-1 (MOI = 0.5) for 1 h followed by twice PBS wash and culture in full medium for 24 h. Data are representative of three (a-b) or two independent experiments (c–f). Graphs show mean ± S.D. (n = 4 for (a, b, f) or n = 3 for (c), biologically independent experiments). Statistical significance was determined using two-tailed Student’s t tests in (a–c, f). The quantitative anaylsis is derive from the same experiment and that blots were processed in parallel in (d). Source data are provided as a Source Data file.