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P. carinii PHR1 exhibits pH-regulated mRNA expression. To examine whether P. carinii PHR1 expression responded to environmental pH, organisms were freshly isolated and maintained in medium at the indicated pH prior to isolation of RNA and Northern analysis. Optimal steady-state mRNA expression of P. carinii PHR1 was observed at physiological pH of 7.0 to 7.5. RNA loading was verified by ethidium bromide staining and reprobing of the blot with P. carinii actin.
