FIG. 4.

P. carinii PHR1 complements growth of S. cerevisiae gas1 mutants in the presence of SDS. S. cerevisiae gas1 mutants lack the ability to grow in the presence of the osmotic destabilizing agent SDS due to cell wall instability. The Gas1p-deficient mutants were cultured following transformation with vector alone, with the wild-type gene present in YEp-GAS1 (positive control), or with the vector containing P. carinii PHR1 (p245GAL-PHR1). Identical plates were cultured in (A) selective minimal medium or (B) minimal plates supplemented with 0.005% SDS. P. carinii PHR1 successfully rescued the cell wall instability of the yeast mutant and restored growth in the presence of SDS.