FIGURE 2.

Evaluation of hCB₂R-dependent inhibition of FSK-stimulated cAMP. CHO cells stably-expressing hCB₂R were treated with 0.10 nM—10 μM compounds and 10 µM FSK for 90 min and cAMP inhibition was measured. (A) Parent compounds used for the design of the JR derivatives; (B,C) compounds of series A; (D) compounds of series B. CP55,940 data are the same in all four panels and presented for reference. Data are expressed as % CP55,940 response. Data were fitted to a non-linear regression (3 parameter model, GraphPad v. 9.0). Data are mean ± S.E.M. of six independent experiments performed in triplicate.