TABLE 2.

[³H] CP55,940 binding.

Compound(s)	hCB1R		hCB2R
	IC50 (nM) (95% C.I.)	E min (% CP55,940) ± S.E.M	IC50 (nM) (95% C.I.)	E min (% CP55,940) ± S.E.M
CP55,940	6.6 (2.7–15)	0.0 ± 5.6	40 (7.7–220)	1.5 ± 14.3
EC21a	n.c	103 ± 2.5***	36 (4.6–77)	115 ± 7.9***
LV62	n.c	106 ± 3.6***	0.65 (0.13–34)	16 ± 11.9
JR14a	n.c	98 ± 2.5***	1.8 (0.26–6.2)	59 ± 12.3*
JR64a	n.c	104 ± 4.7***	0.60 (0.20–26)	20 ± 10.3

Competition binding of [³H]CP55,940 to hCB₁R and hCB₂R was quantified in membranes derived from CHO-K1 hCB1R or hCB2R cells incubated with compounds for 2 h. Data were fit to a three-parameter non-linear regression in GraphPad (v. 9.0). Data are mean with 95% C.I. (IC₅₀) or mean ± S.E.M, n ≥ 3 independent experiments performed in duplicate. Statistical analyses were by non-overlapping C.I. or two-way ANOVA followed by Bonferroni’s post-hoc test. *p < 0.05, ***p < 0.001 relative to CP55,940 within receptor. Data from this Table are graphed in Figure 4 n. c, not converged. E _min values for data that were not fit to a non-linear regression are the mean from the minimum value observed.