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. 2022 Sep 27;12:1004183. doi: 10.3389/fcimb.2022.1004183

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Copyright © 2022 Sun, Jia, Huang, Xiao, Zhou, Zhang, Fu, Xu, Qu, Cui and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effectiveness of the primer set for the H. influenzae-MCDA reaction. The DNA templates extracted from H. influenzae strains were effectively amplified with MCDA reaction at 65°C, and there is no reaction for the blank controls (DW).