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. 2022 Sep 27;12:1004183. doi: 10.3389/fcimb.2022.1004183

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Copyright © 2022 Sun, Jia, Huang, Xiao, Zhou, Zhang, Fu, Xu, Qu, Cui and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Specificity confirmation for H. influenzae-real-time MCDA assay. DNA templates from 2 H. influenzae strains and 28 non-H. influenzae strains were tested by the real-time MCDA assay. No reactions were recorded within the non-H. influenzae strains (A). The results were also confirmed by MCDA reactions, indicated by visual detection reagent (B) and LFB test (C). (B) Tube/Strips 1 and 2 represented two H. influenzae strains, and the other ones represented non-H. influenzae strains. (C) TL, test line; CL, control line.