TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Azoarcus sp. strain T | Wild type | 12 |
| AST2 | ΔbssA | This study |
| AST3 | AST2 containing pGA14 | This study |
| E. coli | ||
| DH10B | F−mcrAΔ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara leu)7697 araD139 galU galK nupG rpsL λ− | Bethesda Research Laboratories |
| TG1recO 1504::Tn5 | K-12 supE thi Δ(lac-proAB) hsdΔ5 F′ [traD36 proA+B+ lacIqlacZΔM15] | Novagen |
| Plasmids | ||
| pBluescript II KS | ColE1 ori, lacZ, Apr | Stratagene |
| pLAFR5-SK1 | oriV, cos, Tetr | 15 |
| pBJ113 | pUC118 containing Kmr and galK; plasmid used for generating gene replacements, derived from pKG2 | 14, 32 |
| pRK415 | oriV, oriT, Tetr | 15 |
| p810F | 25-kb Sau3A fragment of partially digested chromosomal DNA of Azoarcus sp. strain T in pLAFR5-SK1 | This study |
| pGA1 | 5.3-kb HindIII fragment of 25-kb insert of p810F in pBluescript-II KS | This study |
| pGA6 | 2.6-kb PCR deletion of pGA1 in pBJ113; ΔbssA | This study |
| pGA11 | 4.2-kb XbaI/HindIII fragment of 25-kb insert of p810F in pBluescript-II KS | This study |
| pGA14 | 5.3-kb insert of pGA1 in pRK415 | This study |