FIG. 3.

Time course of production of the 40-kDa protein of S. aureus Newman in BHI. A total of 200 ml of BHI was inoculated with 2 ml of an overnight culture in the same medium and incubated at 37°C with shaking. Samples were taken after 2 h and subsequently each hour to monitor the OD₆₅₀ nm of growing culture and to extract cell surface proteins with 2% SDS. Panels display the growth curve (upper left), SDS-PAGE gels of surface protein extracts (lower left), and Western ligand blots of surface protein extracts incubated with biotinylated Fn (upper right) and Fg (lower right). Arrows indicate the 40-kDa protein.