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Growth and primer extension analysis of algD. (A) Growth of ATCC 9046 (solid circles) and JM3 strains (empty circles) in Burk's sucrose medium. (B) Primer extension at 8 (lane 1), 24 (lane 2), and 48 h (lane 3) incubation in Burk's sucrose medium. (C) Hybridization of a sample of the RNA (10 μg) used as template for the primer extension with a probe specific for 16S rRNA over 8, 24, and 48 h (4).
