TABLE 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| A. vinelandii strains | ||
| ATCC 9046 | Highly mucoid, wild type | ATCC |
| JM3 | ATCC 9045 with a gacA::Gm mutation | This work |
| CNS59 | ATCC 9046 with a rpoS::Sp mutation | This work |
| E. coli strain | ||
| DH5α | supE44 ΔlacU169 hsdR17 recA1 endA1 gyrA96 hi-1 relA1 | GIBCO-BRL |
| Plasmids | ||
| pBluescript KS(+) | Stratagene | |
| pKT230 | Broad-host-range vector, Kmr Smr | 2 |
| pHP45Ω-Sp | 14 | |
| pBSL141-Gm | 1 | |
| pSMU1886 | pCP13 cosmid vector containing 25 kb of A. vinelandii DNA, including gacA | This work |
| pSAFA1 | pBluescript KS(+) carrying the gacA gene cloned by PCR | This work |
| pSAFA2 | pBluescript KS(+) carrying a 3.0-kb ClaI fragment with gacA | This work |
| pSAFA3 | pSAFA2 derivative with a gacA::Sp mutation | This work |
| pSAFA4 | pKT230 vector carrying the gacA gene cloned by PCR | This work |
| pCNS59 | pBluescript KS(+) carrying a 0.8-kb fragment with rpoS | This work |
| pSMS7 | pCNS59 derivative with an rpoS::Sp mutation | This work |