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PA overload promotes apoptosis in SCs. (A, B) mRNA expression levels of smpd3 and cls1 in PA-treated SCs. (C–E) After PA treatment at concentrations of 0 mM and 0.5 mM for 24 h, the apoptosis rate of SCs was detected by flow cytometry (FCM). (F) Apoptosis-related protein expression in PA-treated SCs. 7-AAD, 7-aminoactinomyosin D. Data were expressed as mean ± SD, *p < 0.05, **p < 0.01.
