TABLE 2.
Primers used to construct the PrrB mutants
| Plasmid | Primer | Nucleotide sequence (5′–3′)a | Insertion or deletion site in PrrB |
|---|---|---|---|
| pLP1 | LP1+ | TGGGGGTCCGCGCGGCGGCCGCAGCGCTGCCGATGGGGCTCTGCTTC | Insertion of five Ala residues between R48 and L49 |
| LP1− | CCCATCGGCAGCGCTGCGGCCGCCGCGCGGACCCCCAGATACCAGTC | ||
| pLP2 | LP2+ | TCTTCCCGCAGGCGGCGGCAGCCGCGAACCGCCGCCTGACCGAGTTC | Insertion of five Ala residues between Q74 and N75 |
| LP2− | AGGCGGCGGTTCGCGGCTGCCGCCGCCTGCGGGAAGACGAAGGTCGC | ||
| pLP3 | LP3+ | TCCTGCTGTTCGCGGCGGCCGCAGCGCTGACCGGGGGGCTCACCAAC | Insertion of five Ala residues between F99 and L100 |
| LP3− | CCCCCGGTCAGCGCTGCGGCCGCCGCGAACAGCAGGAACGACAGCTG | ||
| pLP4 | LP4+ | TCGAGCTGCGCGCGGCGGCAGCCGCGACGACCGTCATTCTCGGGGCC | Insertion of five Ala residues between R126 and T127 |
| LP4− | ATGACGGTCGTCGCGGCTGCCGCCGCGCGCAGCTCGAGCGCGAGCGC | ||
| pLP5 | LP5+ | ACGGGTCGAGCGCGGCGGCCGCAGCGCTGTCCGTCCCGCGCATGTTC | Insertion of five Ala residues between S156 and L157 |
| LP5− | GGGACGGACAGCGCTGCGGCCGCCGCGCTCGACCCGTCGGCGAGGAT | ||
| pTM1Δ | TM1+ | GACTGCGCACCCTGATCCTCACCGAGTTCCAGGCGCTGAT | Deletion from L26 to L78 |
| TM1− | ATCAGCGCCTGGAACTCGGTGAGGATCAGGGTGCGCAGTC | ||
| Outer primer | OUT+ | ACCATGATTACGCCAAGCTTG | |
| OUT− | TTGATCGTGGCCAGCGGCGTG |
a
Inserted nucleotides are shown in boldface.