TABLE 3.
Levels of spectral complexes in R. sphaeroides strains grown under anaerobic and aerobic conditionsa
| Strain | Plasmid | Level of indicated complex. (nmol/mg of protein) in:
|
|||
|---|---|---|---|---|---|
| Dark-DMSO
|
30% O2
|
||||
| B800–850 | B875 | B800–850 | B875 | ||
| 2.4.1 | pRK415 | 20.23 ± 1.72 | 5.74 ± 0.21 | 0.05 ± 0.00 | 0.02 ± 0.01 |
| PrrB1 | pRK415 | 1.36 ± 0.14 | 5.52 ± 0.27 | 0.06 ± 0.02 | 0.36 ± 0.12 |
| PrrB1 | pPRRB4 | 21.13 ± 0.26 | 7.73 ± 0.11 | 0.11 ± 0.01 | 0.74 ± 0.01 |
| PrrB1 | pLP1 | 24.83 ± 1.86 | 8.67 ± 0.61 | 0.40 ± 0.08 | 3.66 ± 0.09 |
| PrrB1 | pLP2 | 19.12 ± 0.27 | 8.42 ± 0.21 | 0.53 ± 0.09 | 2.95 ± 0.11 |
| PrrB1 | pLP3 | 9.39 ± 0.11 | 6.57 ± 0.06 | n.d. | n.d. |
| PrrB1 | pLP4 | 19.15 ± 1.68 | 8.99 ± 0.95 | 0.67 ± 0.09 | 3.60 ± 0.42 |
| PrrB1 | pLP5 | 25.55 ± 0.03 | 9.37 ± 0.26 | 0.42 ± 0.07 | 3.52 ± 0.24 |
| PrrB1 | pTM1Δ | 21.67 ± 0.27 | 10.04 ± 0.14 | 0.55 ± 0.06 | 3.76 ± 0.11 |
a
Strains were grown anaerobically in the dark with DMSO (Dark-DMSO) or aerobically by sparging with 30% O2–69% N2–1% CO2 to an optical density at 600 nm of 0.4 to 0.5. All values are the averages of two independent determinations. n.d., not determined.