Table 3. Screening of ADHs Combined with the Selected EREDs for the One-Pot Cascade Bioreduction of 1aa.
| entry | ERED | ADH | c (%)b | syn/antic | syn-3a ee (%)c | anti-3a ee (%)c |
|---|---|---|---|---|---|---|
| 1 | 110 | LbADH | >99 | 99:1 | 98 (1R,2R) | n.d. |
| 2 | 110 | TeSADH | 98 | 96:4 | 98 (1R,2R) | n.d. |
| 3 | 110 | ADH-T | >99 | >99:1 | 96 (1R,2R) | n.d. |
| 4 | 110 | ADH-A | >99 | 98:2 | 93 (1R,2R) | n.d. |
| 5 | 110 | evo.1.1.200 | >99 | <1:>99 | n.d. | >99 (1S,2R) |
| 6 | 110 | KRED-P2-D03 | >99 | <1:>99 | n.d. | 99 (1S,2R) |
| 7 | 110 | KRED-P3-B03 | >99 | >99:<1 | 91 (1S,2S) | n.d. |
| 8 | P1-H09 | LbADH | >99 | >99:<1 | 85 (1S,2S) | n.d. |
| 9 | P1-H09 | TeSADH | 90 | >99:<1 | 92 (1S,2S) | n.d. |
a
For further details, see Table S9.
b
Determined by GC.
c
Determined by HPLC. Major diastereoisomer in parentheses. n.d.: not determined.