TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Bacterial strains | ||
| Acinetobacter sp. strain ADP1 | Wild-type | 24 |
| Derivatives of strain ADP1 | ||
| ADP1424 | pobR1424 | 26 |
| ADP1451 | pobR1451 | 26 |
| ADP6205 | pcaH9 | 14 |
| ADP6209 | ΔpcaH20 | 14 |
| ADP6314 | pcaH5 | 14 |
| ADP6407 | pcaH12 | 14 |
| ADP6417 | ΔpcaH19 mutS+ | 14 |
| ADP7003 | pcaH+ pcaG+ ΔmutS11::pGEM3Z(+) (Apr) | This study |
| ADP7118 | ΔpcaH20 ΔmutS6::Ω (Str/Spr) | This study |
| ADP7021 | ΔmutS6::Ω (Str/Spr) | This study |
| ADP7037 | ΔpcaH19 ΔmutS6::Ω (Str/Spr) | This study |
| ADP7053 | pcaH5 ΔmutS6::Ω (Str/Spr) | This study |
| ADP7072 | pobR1424 ΔmutS6::Ω (Str/Spr) | This study |
| ADP7073 | pobRI451 ΔmutS6::Ω (Str/Spr) | This study |
| ADP7087 | orf1::Ω (Str/Spr) | This study |
| ADP7088 | ΔfdxA::Ω (Str/Spr) | This study |
| ADP7098 | pcaH12 ΔmutS6::Ω (Str/Spr) | This study |
| ADP7099 | pcaH9 ΔmutS6::Ω (Str/Spr) | This study |
| Wild-type strains | ||
| Acinetobacter sp. strain 93A2 | pcaH+ pcaG+ | 1 |
| Acinetobacter sp. strain AD321 | pcaH+ pcaG+ | This study |
| Acinetobacter sp. strain AD532 | pcaH+ pcaG+ | This study |
| Acinetobacter sp. strain 01B0 | pcaH+ pcaG+ | 1 |
| Acinetobacter sp. strain 48A1 | pcaH+ pcaG+ | 1 |
| A. haemolyticus 40B4 (ATCC 17906) | pcaH+ pcaG+ | 1 |
| A. johnsonii LUH540 | L. Dijkshoorn | |
| Acinetobacter sp. strain AC423D | This study | |
| Pseudomonas putida PRS2000 | pcaH+ pcaG+ | 36 |
| Plasmids | ||
| pGEM 3zf(+) | Cloning vector (Apr) | Promega |
| pHP45Ω | Ω element (Apr) (Str/Spr) | 38 |
| pUI1638 | Ω element (Apr) (Str/Spr) | 10 |
| pZR2 | 2,392-bp fragment containing pcaK′CHG in pUC19 | 14 |
| pZR419 | ΔpobSR::Ω in pUC19 (Str/Spr) (Apr) | Tony DiMarco |
| pZR7000 | 1942-bp PCR fragment of ADP1 mutS inserted in pGEM3Zf(+) (Apr) | This study |
| pZR7006 | pZR7000 with the ΔmutS6::Ω mutation (Kmr) | This study |
| pZR7008 | pZR7000 with the mutS8::Ω mutation (Str/Spr) | This study |
| pZR7009 | 5′ end of ADP1 mutS and 9 kbp of upstream DNA (Apr) | This study |
| pZR7010 | 3′ end of ADP1 mutS and 8 kbp of downstream DNA (Apr) | This study |
| pZR7072 | 1,165-bp PCR fragment, containing orf1, ligated into pGEM3Zf(+) (Apr) | This study |
| pZR7074 | orf1::Ω (Str/Spr) | This study |
| pZR7075 | 1,532-bp PCR product containing a portion of fdxA and downstream DNA, ligated into pGEM3Zf(+) (Apr) | This study |
| pZR7076 | ΔfdxA::Ω (Str/Spr) | This study |