TABLE 2.
Primers used for PCR amplification of Acinetobacter DNA
| Primer | Nucleotide sequencea | Annealing siteb |
|---|---|---|
| MUTC | 5′-GTCAACTGGGCAGACTTCTAC-3′ | 3′ strand of fdxA |
| MUTF | 5′-CATGGTCGATATTTTGCTGAG-3′ | 5′ strand of fdxA |
| MUTRR5 | 5′-GTTTAACGCCGAGACAAG-3′ | 5′ strand of mutS |
| MUTRR8 | 5′-CCAGATGAGTGCATTGACTGC-3′ | 3′ strand of orf3 |
| MUTSF2 | 5′-AYCGXATGGXGAYTTYTAYGARC-3′ | 5′ strand of mutS |
| MUTSR3 | 5′-GCXGTYTCXGTCATYTCXAC-3′ | 3′ strand of mutS |
a
Primers MUTSF2 and MUTSR3 are degenerate primers.
b
Location in the mutS chromosomal region of Acinetobacter sp. strain ADP1.