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. 2022 Sep 27;4:930110. doi: 10.3389/fgeed.2022.930110

FIGURE 2.

FIGURE 2

Generation of Sol8 Gne KO cells. (A), schematic representation of the 3 gRNAs location in Gne exon 3. (B), sequences of clone 12 and clone 13 DNAs, showing the respective deletions generated by Crisp/Cas9. (C), schematic representation of the location of the primers used to test the cDNA sequences of clones 12 and 13. (D), sequences of the cDNAs from clones 12 and 13 aligned with the respective DNA. (E,F), sequences at the junction regions between the different exons in clone 12 and clone 13 respectively.