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. 2022 Sep 27;4:930110. doi: 10.3389/fgeed.2022.930110

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Copyright © 2022 Ilouz, Harazi, Guttman, Daya, Ruppo, Yakovlev and Mitrani-Rosenbaum.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the GNE KO status in Sol8 manipulated cells. (A), schematic representation of the location of the primers used to verify Gne KO homogeneity; (B), agarose gels illustrating the PCR products of clones 12 and 13 (yellow arrows) compared to controls (blue arrows); (C), immunostaining of Sol8 Gne KO cells with anti-GNE Ab. (D), FACS analysis of PSA positive cells (% of positive cells) (n = 4 replicates); (E), Incucyte confluency analysis; (F), cell size determination (n > 300 cells); (G), FACS analysis of anti Ki67 Ab immunostained Sol8 cells (% of positive cells) (n = 3 replicates).