TABLE 1.
Purification of DL-FalDH from M. capsulatus Bath by method I
Step | Total protein (mg) | Total activity (U) | Sp act (U per mg of protein) | Yield (%) | Purification (fold) |
---|---|---|---|---|---|
Membrane | 15,042 | 19.0 | 1.3 | 100 | 1 |
Detergent extract | 14,892 | 18.9 | 1.3 | 100 | 1 |
DEAE-Sepharose | 909 | 6.2 | 6.8 | 33 | 5 |
Phenyl-Sepharose | 428 | 4.9 | 11.5 | 26 | 9 |
Resource Q | 26 | 3.8 | 146.0 | 20 | 116 |