FIGURE 4.

Inhibition of p70S6K attenuates reconsolidation of cocaine contextual memory. (A) Condition place preference was established with cocaine 10 mg/kg in three groups of mice as shown on day 9 during a 30-min test session. Mice were re-exposed to the cocaine-paired context in a drug-free state for 10 min to reactivate cocaine-associated memories on day 10. The p70S6K inhibitor, PF-4708671 (10 or 50 mg/kg ip), or vehicle was administered immediately after exposure to the cocaine compartment. Mice were re-tested for cocaine place preference 24 h and 7 d later. PF-4708671 (50 mg/kg) significantly abolished the previously established place preference when tested 24 h and 7 d after administration (days 11 and 18). Mice injected with vehicle maintained a significant cocaine place preference on days 11 and 18. (B) Condition place preference was established with cocaine 10 mg/kg in two groups of mice as shown on day 9. On day 10, mice remained in their home cages in the testing room and were injected with PF-4708671 (50 mg/kg ip) or vehicle. The test for cocaine place preference was repeated 24 h and 7 days later. PF-4708671 administered in the home cage (i.e., no exposure to the cocaine context) did not alter the previously established cocaine place preference; both vehicle and PF-4708671 groups maintained a cocaine place preference when retested on days 11 and 18 in the absence of re-exposure to the cocaine context. **p < 0.01, ***p < 0.001 for drug vs. vehicle at same time point; Data are expressed as means ± SEM. N = 9–10/group.