Fig. 3.

LARRPM repressed LUAD development in vivo. a A549 cells with LARRPM stable overexpression or A549 control cells were injected into tail vein of nude mice. Six weeks later, lung metastatic nodules were detected using hematoxyln and eosin (HE) staining. b, c Immunohistohemistry (IHC) staining of the antigens Ki67 (b) and PCNA (c) in lung metastatic nodules formed by A549 cells with LARRPM stable overexpression or A549 control cells. Scale bar: 50 µm. d TUNEL assays using lung metastatic nodules formed by A549 cells with LARRPM stable overexpression or A549 control cells. Scale bar = 50 µm. e Immunofluorescence (IF) staining of F4/80 antigen in lung metastatic nodules formed by A549 cells with LARRPM stable overexpression or A549 control cells control. Scale bar: 50 µm. Results are shown as the mean ± SD based on n = 6 mice in each group. Asterisks indicate a significant difference at **P < 0.01, ***P < 0.001 by the Student’s t-test