Fig. 6.

LARRPM repressed M2 macrophage infiltration. a Schematic diagram of in vitro co-culture system of phorbol-12-myristate-13-acetate (PMA)-stimulated human monocyte line THP-1 cells and LUAD cells. b THP-1 cells were subjected to transwell migration assays towards A549 cells with LARRPM overexpression or A549 control cells. Scale bar: 100 µm. c THP-1 cells were subjected to transwell migration assays towards HCC827 cells with LARRPM depletion or HCC827 control cells. Scale bar: 100 µm. d Expression of M1 and M2 polarization markers in THP-1 cells co-cultured with A649 cells with LARRPM overexpression or A549 control cells was detected by qRT-PCR. e Expression of M1 and M2 polarization markers in THP-1 cells co-cultured with HCC827 cells with LARRPM depleted or HCC827 control cells was detected by qRT-PCR. f, g CD163 (f) and CD206 (g) IF staining in THP-1 cells co-cultured with A549 cells with LARRPM overexpression or A549 control cells, or THP-1 cells co-cultured with HCC827 cells with LARRPM depleted or HCC827 control cells. Results are shown as the mean ± SD based on three independent experiments. Asterisks indicate a significant difference at *P < 0.05, **P < 0.01, ***P < 0.001, by Student’s t-test (b, d) or one-way ANOVA followed by Dunnett's multiple comparisons test (c, e). ARG1 Arginase 1, IL interleukin, TNF tumor necrosis factor