Fig. 7.

LARRPM repressed CSF1 expression via inducing DNA methylation. a, b The expression of cytokines related to macrophage recruitment and polarization in A549 cells with LARRPM overexpression (a) and HCC827 cells with LARRPM depletion (b) was detected by qRT-PCR. c The expression of CSF1 in another LARRPM stably depleted HCC827 clone was detected by qRT-PCR. d, e ChIP assays followed by qPCR were performed in A549 cells with LARRPM overexpression (d) and HCC827 cells with LARRPM depletion (e) to detect the DNAs (LINC00240 promoter) bound by TET1. f, g 5hmC levels of the CpG island CpG82 from A549 cells with LARRPM overexpression (f) and HCC827 cells with LARRPM depletion (g) were measured using the EpiMark 5-hmC Analysis Kit. h, i DNA methylation levels of CpG82 from A549 cells with LARRPM overexpression (h) and HCC827 cells with LARRPM depletion (i) were measured using bisulfate DNA sequencing. j, k CSF1 secretion from A549 cells with LARRPM overexpression (j) and HCC827 cells with LARRPM depletion (k) was measured by enzyme-linked immunosorbent assay (ELISA). Results are shown as the mean ± SD based on three independent experiments. Asterisks indicate a significant different at *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant, by Student’s t-test (a–d, f, h, j) or one-way ANOVA followed by Dunnett's multiple comparisons test (e, g, i, k). CCL2, -3, -4 CC chemokine family, CSF1, CSF2 Colony Stimulating Factor 1, IFN interferon, TGF tumor growth factor