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. 2022 Oct 11;22:244. doi: 10.1186/s12866-022-02658-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — A Antibiofilm assay by test tube method. The first tube in all images represents a positive control. The second and third tubes represent biofilm formed by bacterial cells in presence of antibiotic and ZnO-NPs; respectively. The fourth tube represents the biofilm formed by bacterial cells in presence of a combination of both antibiotic and ZnO-NPs. A; Gentamicin, B; Azithromycin, C; Cefotaxime, D; Ciprofloxacin, E; Clindamycin, and F; Chloramphenicol. B A combination between ZnO-NPs and antibiotics reduces S. aureus biofilm formation. Bacteria biofilms were stained with 1% crystal violet and percentage of biofilm inhibition was analyzed spectrophotometrically. Results are expressed as mean ± SE of three independent experiments. * P < 0.05 was considered significant using 2way ANOVA test