Table 2.
List of antibodies used for Western blots
| Antibody | Concentration | Manufacturer | Catalog No |
|---|---|---|---|
| Unconjugated primary antibodies | |||
| B-arrestin | 1:100 | ThermoFisher, Waltham, MA, USA | 39-5000 |
| p38 | 1:500 | Abcam, Cambridge, UK | ab31828 |
| Dynorphin | 1:100 | Abcam, Cambridge, UK | ab82509 |
| ERK 1/2 | 1:500 | Cell Signaling, Danvers, MA, USA | 9102S |
| Kappa Opioid Receptor | 1:50 | ThermoFisher, Waltham, MA, USA | 44-302G |
| 1:1000 | |||
| Secondary antibodies | |||
| IRDye 680RD Goat Anti Mouse | 1:2000 | LI-COR Biosciences, Lincoln, NE | 926-32210 |
| IRDye 800CW Goat Anti Rabbit | 1:2000 | LI-COR Biosciences, Lincoln, NE | 827-08365 |