Skip to main content
. 2022 Sep 27;12:929123. doi: 10.3389/fonc.2022.929123

Figure 4.

Figure 4

MYCN amplification induces sensitivity to prexasertib treatment in 11q wild type cells. (A) Dose-response curves of two 11q deleted cell lines with MNA (dark blue; NGP and SJNB-6) and two MYCN wild type cell lines (light blue; CHLA-90 and SKNAS) following 72-hour treatment with prexasertib. (B) Dose-response curves of two 11q wild type cell lines with MNA (red; SKNBE and SJNB-8) and two MYCN wild type cell lines (orange; SH-SY5Y and SJNB-12) following 72-hour treatment with prexasertib. Average dose-response curves are indicated in bold, and all curves represent the average of replicates (n=2) where error bars indicate the standard error of the mean (SEM). (C) Western blot of N-myc expression for Shep21n and Shep2 cells without tetracycline treatment and for Shep21n with 50 ng/ml tetracycline treatment for 24, 48 and 72 hours. (D) Dose-response curves for 72-hour prexasertib treatment in the cell lines Shep2 (orange), Shep21n (red) and Shep21n with 50 ng/ml tetracycline (grey).