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Proteolytic activity of MMP-9 by gelatin zymography and MMP-9 protein expression in colonic tissue during iodoacetamide-induced UC in rats (A) and IL-10 KO mice (B). Gelatinolytic bands of MMP-9 are represented as white bands. Protein levels in colonic tissue were determined by Western blot. GAPDH levels were used as loading controls. Assays were repeated three times with highly reproducible results using protein from 3 animals/group.
