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. 2022 Sep 15;88(19):e01276-22. doi: 10.1128/aem.01276-22

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FIG 7 — The effects of metal cations on λ DNA activity of recombinant AS87_RS02955 (rAS87_RS02955). Optimal cation concentrations were measured by incubating rAS87_RS02955 with λ DNA in citric acid-Na₂HPO₄ buffer (pH 6.5) for 30 min at 37°C. (A) The effects of Mg²⁺ on λ DNA cleavage activity of rAS87_RS02955. Optimal activity ranged from 20 to 60 mM. (B) The effects of EDTA on λ DNA cleavage activity of rAS87_RS02955. Enzymatic activity was inhibited by 0.5 mM EDTA and completely inhibited at 25 mM. (C) The effects of Na⁺ on λ DNA cleavage activity of rAS87_RS02955. Optimal rAS87_RS02955 activity was determined at 100 mM. (D) The effects of K⁺ on λ DNA cleavage activity of rAS87_RS02955. Optimal rAS87_RS02955 activity ranged from 100 to 250 mM.