TABLE 1.
Strains used in this study
| Strain | Name or type used in this study | Genotype | Description | Reference or source |
|---|---|---|---|---|
| E. coli | GB05RedTrfA | DH10B fhuA::IS2 ΔybcC ΔrecET PRhaSR-γβαA pBAD-trfA | Strain used for Red/ET recombineering | 55 |
| GBdir-gyrA462 | DH10B fhuA::IS2, ΔybcC ΔrecET PBAD-ETγA gyrAArg462Cys | Arg462Cys mutation in GyrA subunit of DNA gyrase conferring CcdB resistance | 55 | |
| BL21(DE3) | F– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7 p07 ind1 sam7 nin5]) [malB+]K-12(λS) | Strain used as heterologous host of T6SS | Lab stock | |
| MG1655 | K-12 F– λ– ilvG mutant rfb-50 rph-1 | Strain used for competition assay | Lab stock | |
| WM6026 | lacIq rrnB3 ΔlacZ4787 hsdR514 ΔaraBAD567 ΔrhaBAD568 rph-1 attl::pAE12(ΔoriR6K-cat::Frt5) ΔendA::Frt uidA(ΔmluI)::pir attHK::pJK1006Δ(oriR6K-cat::Frt5 trfA::Frt) | Strain used for conjugation, diaminopimelic acid auxotroph | Mekalanos lab | |
| PIR1 | F− Δlac169 rpoS(Am) robA1 creC510 hsdR514 endA recA1 uidA(ΔmluI)::pir-116 | Strain used for cloning | Invitrogen | |
| DH5α | F− ϕ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 phoA supE44 thi-1 gyrA96 relA1 λ− | Strain used for cloning and gene expression | Invitrogen | |
| DAEC 2787 | Strain used for competition assay | Lab stock | ||
| ETEC H-10407 | Strain used for competition assay | Lab stock | ||
| A. dhakensis SSU | WT | Parental strain | Parental strain | 24 |
| ΔvasK | ΔvasK | T6SS null, in-frame deletion of vasK | 24 | |
| ΔtseIc tsiI | ΔtseIc tsiI | In-frame deletion of toxin-coding sequence of tseI and tsiI | 36 | |
| ΔtseIc tsiI ΔvasK | ΔtseIc tsiI ΔvasK | In-frame deletion of vasK in ΔtseIc tsiI background | This study | |
| VipA-sfGFP | vipA-sfgfp | Chromosomal VipA-sfGFP fusion | This study | |
| VipA-N3-sfGFP | vipA-N3-sfgfp | Chromosomal VipA-N3-sfGFP fusion, noncontractile sheath | This study | |
| VipA-sfGFP-NP | vipA-sfgfp | NheI sites inserted flanking T6SS cluster, upstream NheI site inserted in front of native promoter of hcp1 | This study | |
| VipA-sfGFP-Tet | vipA-sfgfp | NheI sites inserted flanking T6SS cluster, upstream NheI site inserted in front of RBS of hcp1 | This study | |
| VipA-N3-sfGFP-NP | vipA-N3-sfgfp | Chromosomal VipA-N3-sfGFP fusion, noncontractile sheath, NheI sites inserted flanking T6SS cluster, upstream NheI site inserted in front of native promoter of hcp1 | This study | |
| VipA-N3-sfGFP-Tet | vipA-N3-sfgfp | Chromosomal VipA-N3-sfGFP fusion, noncontractile sheath, NheI sites inserted flanking T6SS cluster, upstream NheI site inserted in front of RBS of hcp1 | This study | |
| VipA-sfGFP-NP_eff | vipA-sfgfp tseIHFH-AAA tsePE663A | Chromosomal mutations of TseI catalytic residues H1507, F1508, and H1509 and TseP catalytic residue E663; NheI sites inserted flanking T6SS cluster; upstream NheI site inserted in front of native promoter of hcp1 | This study | |
| VipA-N3-sfGFP-NP_eff | vipA-N3-sfgfp tseIHFH-AAA tsePE663A | Chromosomal VipA-N3-sfGFP fusion, noncontractile sheath; chromosomal mutations of TseI catalytic residues H1507, F1508, and H1509 and TseP catalytic residue E663; NheI sites inserted flanking T6SS cluster; upstream NheI site inserted in front of native promoter of hcp1 | This study | |
| VipA-sfGFP-Tet_eff | vipA-sfgfp tseIHFH-AAA tsePE663A | Chromosomal mutations of TseI catalytic residues H1507, F1508, and H1509 and TseP catalytic residue E663; NheI sites inserted flanking T6SS cluster; upstream NheI site inserted in front of RBS of hcp1 | This study | |
| VipA-N3-sfGFP-Tet_eff | vipA-N3-sfgfp tseIHFH-AAA tsePE663A | Chromosomal VipA-N3-sfGFP fusion, noncontractile sheath; chromosomal mutations of TseI catalytic residues H1507, F1508, and H1509 and TseP catalytic residue E663; NheI sites inserted flanking T6SS cluster; upstream NheI site inserted in front of RBS of hcp1 | This study | |
| V. cholerae | C6706 | Strain used for competition assay | Lab stock | |
| V. parahaemolyticus | B5 | Strain used for competition assay | Lab stock | |
| C. rodentium | Strain used for competition assay | Lab stock | ||
| K. pneumoniae | Strain used for competition assay | Lab stock | ||
| P. aeruginosa | PAO1 | Strain used for competition assay | Lab stock | |
| P. asymbiotica | Strain used for competition assay | Lab stock | ||
| P. syringae pv. syringae | Strain used for competition assay | Lab stock | ||