TABLE 1.
Plasmids used in this studya
| Name | Description | Vector | Source or reference |
|---|---|---|---|
| pCM66T | Broad-host-range vector (gift from Mary Lidstrom); ptac IncP ColE1 | NA | Addgene plasmid 74738 |
| pETTF11 | Protein expression plasmid, His6-3c tag, T7 inducible promoter | NA | 94 |
| pmCRc | Codon-optimized mCherry | pEX | Eurofins Genomics |
| pCMF130 | RcGTA promoter fused to codon-optimized mCherry | pCM66T | This study |
| pCMF151 | puf promoter fused to SB1003 RcGTA g16 gene | pCM66T | This study |
| pCMF152 | puf promoter fused to SB1003 cysE1 gene | pCM66T | This study |
| pCMF278 | puf promoter only control | pCM66T | This study |
| pDS1 | cysE1 gene and flanking DNA | pCM66T | This study |
| pDS2 | g16 gene and flanking DNA | pCM66T | This study |
| pDS3 | cysE1::Gent | pCM66T | This study |
| pDS4 | g16::Gent | pCM66T | This study |
| pDS5 | g16-cysE1::Gent | pCM66T | This study |
| pDS6 | cysE2 gene and flanking DNA | pCM66T | This study |
| pDS7 | cysE2::Gent | pCM66T | This study |
| pDS8 | cysE2::Kan | pCM66T | This study |
| pCMF140 | His6-CysE1 | pETTF11 | This study |
| pDS9 | His6-CysE2 | pETTF11 | This study |
a
NA, not applicable.