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Analysis of MecI and BlaI interactions with the yeast two-hybrid systema
| Plasmids | β-Galactosidase activityb |
|---|---|
| pAS1-mecI + pACT2-mecI | 276 ± 69 |
| pAS1-blaI + pACT2-blaI | 287 ± 127 |
| pAS1-mecI + pACT2-blaI | 300 ± 70 |
| pAS1-mecI + pACT2 | 55 ± 19 |
| pAS1 + pACT2-blaI | 47 ± 17 |
| pAS1 + pACT | 11 ± 4 |
Reporter strain Y190 was transformed with the indicated plasmids.
Nanomoles of ONPG hydrolyzed per minute per milligram of protein. Means of at least two assays ± standard deviation.
