TABLE 5.
Complementation of mecA repression and induction with homologous and heterologous mec regulatorsa
| Strain | Relevant genotype | β-Galactosidase activity
|
Fold inductionb | |
|---|---|---|---|---|
| No inducer | CBAP (10 μg/ml) | |||
| GO450TF::630ΔRI | mecR1 mecI+ (c) | 254 ± 40 | 285 ± 24 | 1 |
| GO450TF::630ΔRI + pGO681 | mecR1 mecI+ (c) | 286 ± 37 | 2,824 ± 630 | 10 |
| mecR1+ ΔmecI (p) | ||||
| GO450TF::630ΔRI + pGO682 | mecR1 mecI+ (c) | 318 ± 45 | 345 ± 31 | 1 |
| blaR1+ ΔblaI (p) | ||||
| GO450TF::630ΔI + pGO641 + pCH1988 | mecR1 ΔmecI (c) | 198 ± 43 | 15,639 ± 5,016 | 79 |
| blaR1+ ΔblaI (p) | ||||
| blaR1 blaI+ (p) | ||||
a
See Table 4, footnotes a and b.
b
Value with no inducer divided by value with CBAP at 10 μg/ml.