Figure 1.

sEV characterization

(A) Size and particle concentration of sEVs collected from human umbilical cord blood-derived mononuclear cells (hUCB-MNCs), evaluated by NTA. (B) Zeta potential analysis of sEVs before and after loading with miR-124-3p (miR-124-3p sEVs). (C) Representative TEM images of native sEVs and sEVs enriched with miR-124-3p. Scale bar: 200 nm. (D) Common native sEV markers (CD63, CD9, and HSP70) and potential contaminants (calnexin and ApoA-1) were further analyzed by western blot, where each lane represents a different donor (n = 2). (E) Non-EV markers were found in hUCB-MNC samples but not in (D) native sEV samples. One donor was used. For all other analyzes, n = 3 biological replicates. Statistical significance was calculated using two-way ANOVA with Sidak’s correction, ∗∗∗∗p < 0.0001. sEV, small extracellular vesicles; NTA, nanoparticle tracking analysis; miR-124-3p sEV, miR-124-enriched small extracellular vesicles; TEM, transmission electron microscopy.