TABLE 2.
Viable cell numbers of S. mutans (BM71) biofilms grown at D of 0.1 h−1 following 40 mM glucose pulse with or without pH control
| Culture conditions | Viable cell number (mean ± SD) of:
|
||
|---|---|---|---|
| 12-h biofilms (106/cm2) | 24-h biofilms (106/cm2) | 5-day biofilms (106/cm2) | |
| G-pulse, pH 7.0a | 8.6 ± 3.8 | 21.5 ± 5.3 | 47.4 ± 8.3 |
| G-pulse, pH 5.2b | 26.5 ± 6.8 | 74.5 ± 21.7 | 176.2 ± 29.4 |
a
Glucose pulse with pH control in which biofilms were taken as unadapted cells.
b
Glucose pulse without pH control (pH was decreasing down to 4.89) in which biofilms were taken as adapted cells for assaying acid killing.