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. 2022 May 10;30(10):3193–3208. doi: 10.1016/j.ymthe.2022.05.006

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Autophagy is required for apoV-mediated angiogenesis in athymic nude mice model

(A) The expressions of LC3 in PBS group, apoV group, and 3-MA + apoV group were evaluated by western blots. (Bi–Di) Representative microscope images of H&E staining show regeneration of dental-pulp-like tissue in tooth scaffolds. Scale bars, 1 mm. (Bii–Dii) Enlarged view of boxed area in (i). Scale bars, 100 μm. (Biii–Diii) Immunofluorescence analysis shows the expression of blood vessel marker CD31, with positive represented by green stains. Scale bars, 50 μm; bv, blood vessels; d, dentin.