Figure 2.

Lentivirus transfection of circRNA422 in BMSCs

(A) In the MOI 30 group, the ratio of FITC-positive cells to live cells was 88.6%. (B and C) Quantitative real-time PCR and PCR analyses showed that circRNA422− and circRNA422+ significantly knocked down and upregulated expression of circRNA422 (n = 3, ∗p < 0.05). (D) Alamar blue analysis detecting the optical density (OD) value of the four groups (Con313, circRNA422−, Con510, and circRNA422+) showed that circRNA422− inhibited BMSC proliferation on day 7 and that circRNA422+ promoted BMSC proliferation on days 3 and 7 (n = 3, ∗p < 0.05).