Skip to main content
. 2022 Sep 27;13:910705. doi: 10.3389/fimmu.2022.910705

Figure 4.

Figure 4

SMRT KD cDC1 increased perforin, granzyme, and IFN-γ production ex vivo in CD8 T lymphocytes. (A) Contour plot showing proliferated CD44 effector OT-I T-cells co-cultured with control and SMRT KD cDC1 pulsed with OVA 257-264 peptide overnight followed by CpG challenge. (B) Scatter dot plot with bar showing percent positive of proliferated CD44 effector OT-I T-cells co-cultured with control and SMRT KD cDC1 pulsed with OVA 257-264 peptide overnight followed by CpG challenge (n=12). (C) Contour and scatter dot plots depicting percent positive cells expressing IFNγ, granzyme-B, and perforin by OT-I T-cells co-cultured with CpG stimulated control and SMRT KD cDC1. Gating of the individual markers are determined based on their respective FMO controls (n=8). *p ≤ 0.05, **p ≤ 0.01 and ***p ≤0.001. p-value has been calculated using two tailed unpaired student’s t-test. Data shown in figure is combined from three independent experiments (A, B), four independent replicates (C). Error bars represent SEM.