Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-IRF3 (D9J5Q) | Cell Signaling Technology | Cat# 109495; |
| Rabbit polyclonal anti-PQBP1 | Bethyl Laboratory | Cat# A302–802A |
| Mouse monoclonal anti-PQBP1 | Santa Cruz Biotechnology | Cat# sc-376039; RRID:AB_10989350 |
| Mouse monoclonal anti-PQBP1 (1A11) | Sigma Aldrich | Cat# SAB1402496; RRID:AB_10640643 |
| Rabbit polyclonal anti-cGAS | Novus | Cat# NBP1–8676 |
| Mouse monoclonal anti-cGAS (D-9) | Santa Cruz Biotechnology | Cat# sc-515777; RRID:AB_2734736 |
| Mouse monoclonal anti-cGAS (5G10) | Caymanchem | Cat# 23853 |
| Rabbit monoclonal anti-cGAS (D1D3G) | Cell Signaling Technology | Cat# 49705 |
| Mouse monoclonal anti-GFP (GF28R) | Thermo Scientific | MA5–15256 |
| Rabbit polyclonal anti-GFP | Takarabio | Cat# 632592 |
| Mouse monoclonal anti-Flag | Sigma Aldrich | Cat# F1804; RRID:AB_262044 |
| Human monoclonal anti-HIV-1 p24 (71–31) | AIDS Reagent | Cat# ARP-530 |
| Mouse monoclonal anti-cGAMP | Pfizer (Hall et al., 2017) | PF-07043030 |
| Mouse monoclonal anti-MBP magnetic beads | New England Biolabs | Cat# 28037S |
| Rabbit monoclonal anti-beta-actin (13E5) | Cell Signaling Technology | Cat# 49705 |
| Normal rabbit/mouse IgG | Santa Cruz Biotechnology | Cat# sc-2027; RRID:AB_737197 |
| Bacterial and virus strains | ||
| Human lentiORF cDNA clone of PQBP1 | Open Biosystems (Yoh et. al., 2015) | N/A |
| pNL43 env- luc. | Manel et. al., 2010; Yoh et. al., 2015 | N/A |
| SIV3+ for production of VLP-VPX | D. R. Litman (Manel et. al., 2010; Yoh et. al., 2015) | N/A |
| Gag-IN-mRuby3 | Mamede et al., 2017 | N/A |
| HIV-Gag-iGFP | Mamede et al., 2017 | N/A |
| CypA-dsRed | Francis et al., 2016; Francis and Melikyan, 2018. | N/A |
| Cantell strain of Sendai virus | Mount Sinai Department of Microbiology Virus Collection | N/A |
| Biological samples | ||
| De-identified healthy donor blood | San Diego Blood bank | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| 2’ 3’ -cGAMP | Invivogen | Cat# tirl-nacga23–02 |
| Herring testis DNA | Sigma Aldrich | Cat# D6898 |
| Lipofectamine 2000 | Life Technology | Cat# 11668030 |
| Stemfect RNA transfection kit | ReproCell | Cat# 00–0069 |
| Critical commercial assays | ||
| Duolink in situ detection reagent | Sigma | Cat# DUO92008 |
| Deposited data | ||
| None | N/A | N/A |
| Experimental models: Cell lines | ||
| Dual-KO-cGAS-THP-1 | Invivogen | Cat# thpd-kocgas |
| Dual-KO-cGAS-Flag-cGAS-THP-1 | This paper | |
| THP1-Dual Cells | Invivogen | Cat# thpd-nfis |
| THP-1 | ATCC | Cat# TIB-202 |
| 293T | ATCC | Cat# CRL-3216 |
| Experimental models: Organisms/strains | ||
| N/A | N/A | N/A |
| Oligonucleotides | ||
| siNT (5’-AATCGATCATAGGACGAACGC-3’) | This paper | N/A |
| siPQBP1–1 (5’- AAGCTCAGAAGCAGTAATGCA-3’) | This paper | N/A |
| siPQBP1–2 (5’-AAAGCCATGACAAGTCGGACA-3’) | This paper | N/A |
| Non-targeting guide crRNA guide sequence | Dharmacon | U-007502 |
| MB21D1(cGAS) crRNA guides: 1–5 | Dharmacon | CM-015607-(1 to 5), see Guide RNA sequences under Star Method |
| NONO (4841) targeting guides: 2 and 5 | Dharmacon | CM-007756-(02 and 05), see Guide RNA sequences under Star Method |
| Recombinant DNA | ||
| pEASIL-Flag-cGAS | This paper | N/A |
| pEASIL | M. Malim | N/A |
| pCDFDuet-PQBP1 | This paper | N/A |
| pCDNA 3.1-MBP-cGAS | This paper | N/A |
| Software and algorithms | ||
| Imaris | Bitplane | RRID:SCR_007370 |
|
Python Programming Language
|
Python Software Foundation | RRID:SCR_008394 |
| Custom Scripts for imaging analysis | this article, PMID:33154161, | 10.5281/zenodo.6551125 |
| Other | ||