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. Author manuscript; available in PMC: 2022 Oct 11.
Published in final edited form as: Cell Rep. 2022 Sep 27;40(13):111409. doi: 10.1016/j.celrep.2022.111409

Figure 1. Autophagy genes are HIF targets.

Figure 1.

(A) Principal-component analysis (PCA) of Caco-2 IECs analyzed by ChIP-chip demonstrates association of autophagy genes with hypoxia genes, in contrast with housekeeping genes.

(B) Heatmap of genes plotted in (A) shows conserved patterns between autophagy and hypoxia targets. Sample names at bottom of columns represent biological replicates.

(C) Validation of ChIP-chip results by qPCR in Caco-2 IECs demonstrates induction of autophagy genes under hypoxic conditions.

(D) Analysis of gene expression in primary murine colon-derived organoids by qPCR shows significant increase in autophagy gene expression by hypoxia. *p < 0.05, **p < 0.01 by t test. ChIP-chip results reflect three biological replicates per sample group. qPCR results reflect at least three biological replicates.