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. Author manuscript; available in PMC: 2022 Oct 11.
Published in final edited form as: Cell Rep. 2022 Sep 27;40(13):111409. doi: 10.1016/j.celrep.2022.111409

Figure 4. STm induces cellular Hx in vitro.

Figure 4.

(A) Caco-2 IECs, STm, or a combination of both were incubated at 37°C on Transwell inserts in an Oxodish oxygen measurement system. HBSS+ was used as the assay buffer and as the negative control.

(B) HeLa cells transfected with a plasmid containing luciferase under Hx-response element (HRE) control were infected with STm, then luciferase activity was measured. Both Oxodish and luciferase experiments utilized at least six biological replicates per group.